DefenderPro Predator works around the clock, as long as there is a food source of urine, feces or any organics in the grout, sub floor, walls or other surfaces. Apply Predator during regular cleaning schedules. Predator will penetrate deep into the grout and sub floor where the bulk of the urine is located. The bacteria will “SEEK OUT” the deeply embedded urine. Predator contains an odor counteractant with a light fragrance that can last up to 24 hours. Great for any organic stains and odors.